prolonged and intermittent bilateral common carotid artery occlusion induces brain lipidome changes in a rat stroke model

Authors

masoumeh faezi department of physiology, faculty of biological sciences, shahid beheshti university, g.c., tehran, ir iran

mohammad reza bigdeli department of physiology, faculty of biological sciences, shahid beheshti university, g.c., tehran, ir iran; department of physiology, faculty of biological sciences, shahid beheshti university, g.c., tehran, ir iran. tel: +98-2129902731, fax: +98-2122431664

abstract

objectives in this study, we aimed to investigate the changes in brain lipidomics following prolonged and intermittent ischemic preconditioning. materials and methods two groups underwent bilateral common carotid artery occlusion either intermittently (for 3 continuous periods within a timeframe of 20 minutes, releasing 3 consecutive times, yielding a total of 9 minutes (intermittent bilateral common carotid artery occlusion)) or for 9 minutes continuously (prolonged bilateral common carotid artery occlusion). the third and fourth groups were used as control and sham (without ischemia) groups, respectively. the first three groups were subdivided into middle cerebral artery occlusion-operated (middle cerebral artery occlusion mcao), for 60 minutes of ischemia) and intact (without any surgery) subgroups. after 1 hour of ischemia and 24 hours of reperfusion, the neurologic deficit score (neurological deficit score (nds)) and the infarct volume (infarct volume) were assessed in the middle cerebral artery occlusion-operated subgroup. brain lipidomics were measured in the intact subgroup and the sham group. results preconditioning with prolonged bilateral common carotid artery occlusion and intermittent bilateral common carotid artery occlusion significantly decreased the neurological deficit scores and infarct volume and increased the levels of phosphatidylethanolamine, sphingomyelin, cholesterol ester, cholesterol, phosphatidylcholine, and cerebroside in the brain compared with the control and sham groups. prolonged bilateral common carotid artery occlusion and intermittent bilateral common carotid artery occlusion significantly decreased the brain ceramide and lyso-phosphatidylcholine levels. the triglyceride levels in both groups (intermittent bilateral common carotid artery occlusion and prolonged bilateral common carotid artery occlusion) did not change in comparison with the control and sham groups. conclusions although it seems that further studies are needed to clarify the real mechanisms of ischemic tolerance, ischemic preconditioning could decrease brain ischemia injury via changes in brain lipidomics. background recent studies suggest that prolonged and intermittent bilateral common carotid artery occlusion (prolonged bilateral common carotid artery occlusion (po) and intermittent bilateral common carotid artery occlusion, respectively) can lead to reduced ischemia brain injury in ischemic tolerance.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Prolonged and Intermittent Bilateral Common Carotid Artery Occlusion Induces Brain Lipidome Changes in a Rat Stroke Model

Background: Recent studies suggest that prolonged and intermittent bilateral common carotid artery occlusion (prolonged bilateral common carotid artery occlusion (PO) and intermittent bilateral common carotid artery occlusion, respectively) can lead to reduced ischemia brain injury in ischemic tolerance. Objectives: In this study, we aimed to investigate the changes in brain lipidomics followin...

full text

Bilateral Carotid Artery Occlusion Induces Cochlear Oxidative Stress and Hearing Loss in Rat

Introduction: The aim of this study was evaluation the effects of bilateral carotid artery occlusion on cochlear oxidative stress and hearing status in rat. Methods: The rats were divided into two sets. First set used for electrophysiological recording (click and 4 kHz tone burst ABR and EcochG) at day before surgery and 1st, 4th and 7th days after surgery. Animals of second set that used for ...

full text

Permanent, bilateral common carotid artery occlusion in the rat: a model for chronic cerebral hypoperfusion-related neurodegenerative diseases.

Chronic cerebral hypoperfusion has been associated with cognitive decline in aging and Alzheimer's disease. Moreover, the pattern of cerebral blood flow in mild cognitive impairment has emerged as a predictive marker for the progression into Alzheimer's disease. The reconstruction of a pathological condition in animal models is a suitable approach to the unraveling of causal relationships. For ...

full text

Protective Effects of Quercetin on Rat Pial Microvascular Changes during Transient Bilateral Common Carotid Artery Occlusion and Reperfusion

The aim of this study was to assess the in vivo effects of quercetin on pial microvascular responses during transient bilateral common carotid artery occlusion (BCCAO) and reperfusion. Rat pial microcirculation was visualized by fluorescence microscopy through a closed cranial window. Pial arterioles were classified in five orders of branchings. Capillaries were assigned order 0, the smallest a...

full text

.Neuroprotection induced by Preconditioning with Prolonged and Intermittent Normobaric Hyperoxia Induce Catalase Activity in the rat stroke model

Introduction: Ischemic preconditioning (IPC) is an endogenous phenomenon that can induce ischemic tolerance (IT) in variety of organs such as brain. In this study, we examined the intermittent and prolonged dose of normobaric hyperoxia (HO) on neurologic deficit scores, infarct volume, and catalase activity. Material and Method: The rats were divided to four main groups. First two main groups w...

full text

Common Carotid Artery Occlusion: A Case Series

Subjects and Methods. We analysed 5000 cerebrovascular ultrasound records. A total of 0.4% of the patients had common carotid artery occlusion (CCAO). Results. The mean age was 59.8 ± 14.2 years, and the male/female ratio was 2.33. The most frequent risk factors were hypertension, ischaemic heart disease, dyslipidemia, diabetes mellitus, and smoking. Right-sided and left-sided CCAO occurred in ...

full text

My Resources

Save resource for easier access later


Journal title:
thrita

جلد ۵، شماره ۲، صفحات ۰-۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023